Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser.

نویسندگان

  • David Popp
  • N Duane Loh
  • Habiba Zorgati
  • Umesh Ghoshdastider
  • Lu Ting Liow
  • Magdalena I Ivanova
  • Mårten Larsson
  • Daniel P DePonte
  • Richard Bean
  • Kenneth R Beyerlein
  • Cornelius Gati
  • Dominik Oberthuer
  • David Arnlund
  • Gisela Brändén
  • Peter Berntsen
  • Duilio Cascio
  • Leonard M G Chavas
  • Joe P J Chen
  • Ke Ding
  • Holger Fleckenstein
  • Lars Gumprecht
  • Rajiv Harimoorthy
  • Estelle Mossou
  • Michael R Sawaya
  • Aaron S Brewster
  • Johan Hattne
  • Nicholas K Sauter
  • Marvin Seibert
  • Carolin Seuring
  • Francesco Stellato
  • Thomas Tilp
  • David S Eisenberg
  • Marc Messerschmidt
  • Garth J Williams
  • Jason E Koglin
  • Lee Makowski
  • Rick P Millane
  • Trevor Forsyth
  • Sébastien Boutet
  • Thomas A White
  • Anton Barty
  • Henry Chapman
  • Swaine L Chen
  • Mengning Liang
  • Richard Neutze
  • Robert C Robinson
چکیده

A major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F-actin can be flow-aligned to a disorientation of approximately 5 degrees. Using this XFEL-based technique, we determine that gelsolin amyloids are comprised of stacked β-strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α-synuclein amyloids.

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عنوان ژورنال:
  • Cytoskeleton

دوره 74 12  شماره 

صفحات  -

تاریخ انتشار 2017